您好,歡迎進入上海力敏實業有限公司網站!
一鍵分享網站到:
您現在的位置:首頁 >> 產品中心 >> 生化試劑、抗體、血清 >> 常規生化試劑 >> qiagen301427Effectene Transfection Reagent effectene轉染試劑

Effectene Transfection Reagent effectene轉染試劑

  • 更新時間:  2023-07-24
  • 產品型號:  qiagen301427
  • 簡單描述
  • Effectene Transfection Reagent effectene轉染試劑
    對各種類型的真核細胞DNA轉染,尤其適用于原代細胞
    可在血清存在的情況下進行轉染
    適合用于原代細胞或敏感的細胞系
    高轉染效率而且較低的DNA使用量
    Low endotoxin level with <10 EU/ml
詳細介紹

Effectene Transfection Reagent  effectene轉染試劑

Principle

Effectene Transfection Reagent is an innovative non-liposomal lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids (see figure "Model of Effectene Principle"), providing a particularly efficient way of transferring DNA into eukaryotic cells. Effectene Reagent offers significant advantages over many liposome reagents and other transfection methods (1, 2).

Procedure

The Effectene procedure has two steps. DNA is first mixed with Enhancer and a buffer that provides optimal salt conditions for efficient DNA condensation. This step requires just 2–5 minutes. Effectene Reagent is then added and the mixture is incubated for 5–10 minutes to allow Effectene–DNA complexes to form. The complexes are mixed with growth medium (which can contain serum and antibiotics), and added directly to the cells. The cells are then incubated until harvested and analyzed for gene expression (see flowchart "Effectene Transfection Reagent").

Applications

Effectene Reagent is suitable for transient and stable transfection of a broad range of cell types (see figures "High Transfection Efficiencies Using Effectene Reagent" and "Transfection of Oligonucleotides Using Effectene Reagent"). Because transfection can be performed in the presence of serum and requires low amounts of DNA (see figure "Serum and DNA Quantity vs. Transfection Efficiency"), cytotoxicity is minimal. This makes Effectene Reagent particularly effective for primary cells (see figure "Effectene Reagent with Primary Cells"). A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site — www.qiagen.com/TransfectionTools. View cell lines successfully transfected with this product.

Effectene Transfection Reagent  effectene轉染試劑

High-throughput transfection

The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Reagent requires low amounts of DNA and minimal handling, and in addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Reagent provides outstanding transfection efficiencies, excellent reproducibility, and minimal cytotoxicity in high-throughput transfection, and is available in bulk quantities.

Cited References

1. Ausubel, F.M., et al. eds. (1991) Current Protocols in Molecular Biology, Wiley Interscience, New York.
2. Sambrook, J., Fritsch, E.F., and Maniatis, T., eds. (1989) Molecular cloning — a laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

Images

Model of Effectene Principle High Transfection Efficiencies Using Effectene Reagent Transfection of Oligonucleotides Using Effectene Reagent Effectene Transfection Procedure Serum and DNA Quantity vs. Transfection Efficiency Effectene Reagent with Primary Cells

 

Effectene Transfection Reagent  effectene轉染試劑 詳細產品信息可和選購


留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
主站蜘蛛池模板: 久久综合伊人77777| 人妻少妇精品中文字幕AV蜜桃| 亚洲jizzjizz妇女| 在线精品国产一区二区三区| 一区二区三区欧美在线| 扒开末成年粉嫩的小缝视频| 国产三级精品三级在线专区| 亚洲五月综合缴情婷婷| 国产色在线观看| AAA级久久久精品无码片| 日韩人妻不卡一区二区三区| 亚洲免费人成视频观看| 美女扒开尿口让男人桶免费网站| 国产午夜无码片在线观看| 99久久综合狠狠综合久久aⅴ | 免费体验120秒视频| 精品视频久久久| 国产福利一区二区三区在线观看 | 把胡萝卜立着自己坐上去| 久久亚洲精品国产亚洲老地址| 日韩色图在线观看| 再深点灬舒服灬太大了爽| 老阿姨哔哩哔哩b站肉片茄子芒果| 国产伦理电影网| 韩国三级香港三级日本三级| 国产自产2023最新麻豆| 99国产精品国产精品九九| 天天成人综合网| www.免费在线观看| 日本欧美在线观看| 久久精品无码午夜福利理论片| 最近免费中文字幕大全免费版视频 | 日韩精品人妻系列无码专区免费| 亚洲av无码一区二区三区电影| 欧美丰满熟妇xx猛交| 你是我的女人中文字幕高清 | 国产精品福利一区二区久久| 91情国产l精品国产亚洲区| 国产高清无专砖区2021| 91久久国产情侣真实对白| 国产网站在线免费观看|